ABSTRACT
Objective: To establish a method for determining arabinose, mannose, fructopyranose and amylaceum in Shenxiong glucose injection by UPLC-MS/MS, so as to provide the basis for the scientific evaluation of the quality of Shenxiong glucose injection, and lay a foundation for the safe use of drugs in clinic. Method: Domestic GDX-403 solid-phase extraction column was used to purify Waters ACQUITY UPLC BEH Xbridge Amide column (2.1 mm×100 mm, 1.7 μm)at the column temperature of 35℃, and the mobile phase was 0.1% ammonia, 0.1% acetonitrile-0.1% ammonia water and water 85:15. The contents of arabinose, mannose, fructose and glucose in Shenxiong glucose injection were determined by UPLC-MS/MS with a flow rate of 0.3 mL·min-1. Result: A method was established to determine arabinose, mannose, fructopyranose and amylaceum in Shenxiong glucose injection. The concentration range of arabinose, mannose, fructopyranose and amylaceum showed a good linear relationship with the peak area, with a good repeatability and precision. Recoveries were 98.43%, 102.13%, 100.72%, 101.75%, and RSD were 2.4%, 1.3%, 3.1%, 2.7%. Arabinose and mannose content were stable in five batches of Shenxiong glucose injection. Conclusion: The method is simple and specific. Compared with the determination of total sugar, the method is more scientific and stable, and can be used for the quality control of Shenxiong glucose injection.
ABSTRACT
Compared with the traditional anterior and posterior operation,anterior transpedicular screw fixation (ATPS) has many advantages of hiomechanics, relative safety. Both problems of decompression and reconstruction can be resolved only through an anterior approach. A rather peculiar anatomic channel was used in ATPS, but no special tools was used in system supporting for anterior pedicle screw to place,so the indications of ATPS of lower cervical vertebrae is relatively narrow,it cannot replace of traditional anterior and posterior surgery. Problems of accurately inserting screws and the development of internal fixation device about ATPS is a hot spot of current research and a future direction. In recent years,many scholars have systematically studied the technique, and applied it in clinic gradually and achieved good effects. In order to improve the level of application,recent articles were analyzed retrospectively in this paper,and the studies of anatomy,biomechanical and clinical application of ATPS were reviewed.
Subject(s)
Humans , Biomechanical Phenomena , Bone Screws , Cervical Vertebrae , Wounds and Injuries , General Surgery , Fracture Fixation, Internal , MethodsABSTRACT
<p><b>OBJECTIVE</b>To research the application of the single time detection of HPV E6/E7 mRNA and HC2-HPV-DNA in cervical screening project.</p><p><b>METHODS</b>We detected both HPV E6/E7 mRNA and HC2-HPV-DNA of each sample which collected from 130 cervical disease patients' cervix during Jan. 2008 and July. 2009. TCT results were taken as standard to evaluate the diagnostic accuracy of the above two test methods in detecting high-grade cervical disease.</p><p><b>RESULTS</b>82.3% (107/130)women were confirmed to infect HPV by HC2-HPV-DNA detection, and 40.0% (52/130) women were confirmed to infect HPV by HPV E6/E7 mRNA detection, there was no significant difference between the two results (chi2 = 24.5, P < 0.05). The sensitivity, specificity, positive predictive value, negative predictive value of HC2-HPV-DNA detection were 90.1%, 22.1%, 37.4% and 82.6%, respectively. The sensitivity, specificity, positive predictive value, negative predictive value of HPV E6/E7 mRNA detection were 65.9%, 73.3%, 55.8% and 80.8%, respectively.</p><p><b>CONCLUSION</b>In clinical cervical screening project of single time, the combination of HC2-HPV-DNA detection and HPV E6/E7 mRNA detection wick take on more potential value than applying each of them alone. RNA;</p>